Part:BBa_K1053200

Pconst.-SRⅡ-HtrⅡ-EnvZ-Pompc-HHR-GFP-DT

Halophilic archaea, such as Halobacterium salinarum and Natronobacterium pharaonis (N. pharaonis) are known to show phototaxis by the illumination of light by using receptors called sensory rhodopsin I and II (SRI and SRII). The SR proteins are seven-transmembrane retinylidene photoreceptors, which transmits blue light signal (λmax 487 nm) to their corresponding transducers HtrI and HtrII respectively. This two-component system regulates cells’ flagellar motors for phototaxis (Ref. 1, 2).
In dark, sensory rhodopsin phospholylates OmpR response regulator and OmpR response regulator activate P_ompC, so HHR is expressed. HHR contains GFP’s RBS. When HHR is expressed, HHR causes self cleavage and GFP’s RBS is exposed, so GFP is expressed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 134
  Illegal NgoMIV site found at 392
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1088
  Illegal SapI site found at 862